What is thick and thin film?

Thin film has a thickness in the order of 0.1 micrometer or smaller, while thick film is around thousands time thicker. Thin film resistors have a metallic film that is vacuum deposited on an insulating substrate. Thick film resistors are produced by firing a special paste onto the substrate.

Besides, what is thick and thin blood smear?

A thick blood smear is a drop of blood on a glass slide. Thick blood smears are most useful for detecting the presence of parasites, because they examine a larger sample of blood. A thin blood smear is a drop of blood that is spread across a large area of the slide.

Likewise, how do you make a thick and thin smear? Making thick and thin blood smears Thin film (a): Bring a clean spreader slide, held at a 45° angle, toward the drop of blood on the specimen slide. 3. Thin film (b): Wait until the blood spreads along the entire width of the spreader slide.

Then, what is a thick film?

Thick smears consist of a thick layer of dehemoglobinized (lysed) red blood cells (RBCs). The blood elements (including parasites, if any) are more concentrated (app. 30×) than in an equal area of a thin smear. Thus, thick smears allow a more efficient detection of parasites (increased sensitivity).

Why is it necessary to prepare a thin film smear?

Thin blood films have a low sensitivity for detection of malaria parasites. Therefore they are used in conjunction with thick blood films, in order to identify the malaria species that was detected by microscopic examination of the thick blood film. All blood samples are potentially infectious.

Is it better to have thick or thin blood?

For the heart and circulatory system, though, thinner, more watery blood might be better. Some tantalizing threads of evidence suggest that people with thicker (or more viscous) blood have higher chances of developing heart disease or having a heart attack or stroke.

Why do you avoid the thick area of the smear?

Body and base of the smear These areas are usually too thick to evaluate cells under higher power. The thick area of a smear dries too slowly for leukocytes to spread out.

What is the difference between thick and thin?

adjective, thick·er, thick·est. having relatively great extent from one surface or side to the opposite; not thin: a thick slice.

What are the characteristics of a good smear?

Characteristics of a Good Smear 1) Thick at one end, thinning out to a smooth rounded feather edge. 2) Should occupy 2/3 of the total slide area. 3) Should not touch any edge of the slide. 4) Should be margin free, except for point of application.

Why are blood smears stained?

These stains allow for the detection of white blood cell, red blood cell, and platelet abnormalities. Hematopathologists often use other specialized stains to aid in the differential diagnosis of blood disorders.

What does malaria look like on a blood smear?

It involves collection of a blood smear, its staining with Romanowsky stains and examination of the Red Blood Cells for intracellular malarial parasites. Thick smears are 20–40 times more sensitive than thin smears for screening of Plasmodium parasites, with a detection limit of 10–50 trophozoites/μl.

Why do we use Giemsa stain?

Giemsa stain is used to obtain differential white blood cell counts. It is also used to differentiate nuclear and cytoplasmic morphology of the various blood cells like platelets, RBCs, WBCs. This stain is also used in cytogenetics to stain the chromosomes and identify chromosomal aberrations.

What does a blood smear tell you?

A blood smear is a blood test used to look for abnormalities in blood cells. The three main blood cells that the test focuses on are: red cells, which carry oxygen throughout your body. white cells, which help your body fight infections and other inflammatory diseases.

How do you stain a thin blood film?

Staining procedure for thick blood film Place the thick smear slide flat on a staining rack. Carefully overlay the entire smear with demineralized water. 2. Allow the water to evaporate and the slide to air dry completely.

How do you stain thick smears?

Procedure: • Fill staining dish with staining solution • Place thin film and thick films into the staining dish. Stain blood slides for 45 minutes • Wash in water. Dry it and examine under oil immersion lens of the microscope.

How do you smear blood on the slide?

Place clean glass slide on a flat surface. Add one small drop of blood to one end.
Take another clean slide, and holding at an angle of about 45 deg, touch the blood with one end of the slide so the blood runs along the edge of the slide by capillary action.
Make 2 smears, allow to air dry, and label clearly.

How do you stain malaria parasite?

Thick blood films Field's stain was applied by dipping the slide into Field's stain A for 3 seconds, then into tap water for 3 seconds (with gentle agitation), into Field's stain B for a further 3 seconds and then washing gently in tap water to remove excess stain. The slide was then air dried for at least 30 minutes.

How do you prepare for a peripheral smear?

Well-made peripheral smears can be prepared by starting with only a drop of blood at one end of a clean glass slide. The drop is smeared lightly and quickly with a wedge technique so as to leave a thin "feather" edge where all cells may be examined individually, particularly red blood cells.

How do you prepare Field stain A and B?

Preparation of Field stain B from reagents:

Mix both salts in the water.
Add 500 ml from above into bottle containing glass beads.
Add the stain powders Eosin and mix well.
Then add the remainder of the solution.
Mix well and filter into another clean bottle.
Label the bottle Field stain B, and also write the date.

Why is a thick blood smear considered to have a higher sensitivity than a thin blood smear?

Thick blood smears are more sensitive in detecting malaria parasites because the blood is more concentrated allowing for a greater volume of blood to be examined; however, thick smears are more difficult to read. Thin smears aid in parasite species identification and quantification.

Why is it important to smear the blood as soon as the drop is placed on the slide?

As soon as the drop of blood is placed on the glass slide, the smear should be made without delay. Any delay results in an abnormal distribution of the white blood cells, with many of the large white cells accumulating at the thin edge of the smear.

How do you test for malaria parasite?

Malaria parasites can be identified by examining under the microscope a drop of the patient's blood, spread out as a “blood smear” on a microscope slide. Prior to examination, the specimen is stained (most often with the Giemsa stain) to give the parasites a distinctive appearance.

How do you calculate Parasitemia?

This figure can be converted to the number of parasites per µl of blood; divide the number of parasites per 100 WBCs by 100, and multiply that figure by the number of WBCs per µl of blood. 2. Depending on the parasitemia, 200 or more WBCs may have to be counted, so the denominator may vary (it may be 200 or even more).

How do you test for microfilaria?

Usually, a microfilariae blood test is conducted at night to coincide with the appearance of microfilariae. The microfilaria test procedure requires a blood sample either from the finger prick, earlobe or venous blood. There are a number of tests to identify the presence of microfilariae in one's body.